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KMID : 0613820040140020339
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Journal of Life Science
2004 Volume.14 No. 2 p.339 ~ p.344
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Quantitative Trait Loci for Stem Length in Soybean Using a Microsatellite Markers
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Kim Hyeun-Kyeung
Kang Sung-Taeg
Kong Hyeun-Jong
Choi In-Soo
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Abstract
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Identification of individual quantitative trait loci (QTL) is a prerequisite to application of marker- assisted selection for stem length. Two simple sequence repeat (SSR)-based linkage maps were constructed from recombination inbred line populations between cross of Keunolkong and Shinpaldalkong. Two parents used differed greatly in stem length, which were 30.57 cm and 49.75 cm in Keunolkong and Shinpaldalkong, respectively. Using the constructed maps, regression analysis and interval mapping were performed to identify QTLs conferring stem length. Four QTLs for stem length on linkage groups (LG) F, J, N and O were identified in the Keunolkong×Shinpaldalkong population and they totally explained 37.83% of variation for stem length. In the population, two major QTLs on LG J and O conditioning 14.25% and 10.68% of the phenotypic variation in stem length were determined and two QTLs with minor effect were detected on LG F and N. Identification of QTLs for stem length and mapping individual locus should facilitate to describe genetic mechanisms for stem length in different population. SSR markers tightly linked to QTLs for stem length allow to accelerate the elimination of deleterious genes and selection for desirable recombinants at early stage in crop breeding programs.
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KEYWORD
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Stem length, Soybean, QTLs, Microsatellite marker
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